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A chromosome-level assembly of the seed beetle Callosobruchus maculatus genome with annotation of its repetitive elements

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Erscheinungsjahr: 
2023
Vollständiger Titel: 
A chromosome-level assembly of the seed beetle Callosobruchus maculatus genome with annotation of its repetitive elements
ZFMK-Autorinnen / ZFMK-Autoren: 
Prof. Dr. Alexander Suh
Org. Einordnung: 
Zentrum für molekulare Biodiversitätsforschung (zmb), Molekulare Biodiversität
Publiziert in: 
G3: Genes Genomes Genetics
Publikationstyp: 
Zeitschriftenaufsatz
DOI Name: 
10.1093/g3journal/jkad266
Keywords: 
Chrysomelidae, chromosome conformation capture, X chromosome assembly, transposable elements, Tc1-Mariner
Bibliographische Angaben: 
Arnqvist, G., Westerberg, I., Galbraith, J., Sayadi, A., Scofield, D. G., Olsen, R. A., Immonen, E., Bonath, F., Ewels, P. & Suh, A. (2023). A chromosome-level assembly of the seed beetle Callosobruchus maculatus genome with annotation of its repetitive elements. G3: Genes, Genomes, Genetics, jkad266.
Abstract: 

Callosobruchus maculatus is a major agricultural pest of legume crops worldwide and an established model system in ecology and evolution. Yet, current molecular biological resources for this species are limited. Here, we employ Hi-C sequencing to generate a greatly improved genome assembly and we annotate its repetitive elements in a dedicated in-depth effort where we manually curate and classify the most abundant unclassified repeat subfamilies. We present a scaffolded chromosome-level assembly, which is 1.01 Gb in total length with 86% being contained within the 9 autosomes and the X chromosome. Repetitive sequences accounted for 70% of the total assembly. DNA transposons covered 18% of the genome, with the most abundant superfamily being Tc1-Mariner (9.75% of the genome). This new chromosome-level genome assembly of C. maculatus will enable future genetic and evolutionary studies not only of this important species but of beetles more generally.