The Zoological Research Museum Alexander Koenig

is a research museum of the Leibniz Association

Evaluating the efficiency of different DNA-barcoding approaches for routine stream monitoring tasks

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Project title: 
Evaluating the efficiency of different DNA-barcoding approaches for routine stream monitoring tasks
ZFMK Project lead: 

Description

The focus of this project is to evaluate exemplary for the freshwater fauna of the river Sieg (Germany) the efficiency of different single specimen DNA-barcoding approaches for routine stream monitoring tasks.

The taxonomic resolution of morphological and DNA barcode-based species identification is directly compared, investigating additional value and congruence of both methods. In cases where standard COI barcodes failed to offer unambiguous species identifications, the discrimination capacity of the nuclear rhodopsin marker (RHO) is tested. High resolution melting (HRM) is combined with DNA barcoding (Bar-HRM) to analyse if this identification approach offers a simpler, faster and cheaper alternative to Sanger-sequencing based single specimen barcoding.

Location

Contact person

Head of Centre for Taxonomy and Evolutionary Research
Head of Vertebrate Department
Curator Ichthyology
+49 228 9122-255
+49 228 9122-212
f.herder [at] leibniz-zfmk.de