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Taxonomy assignment approach determines the efficiency of identification of OTUs in marine nematodes

AutorInnen: 
Holovachov, O., Haenel, Q., Bourlat, S. J., Jondelius, U.
Erscheinungsjahr: 
2017
Vollständiger Titel: 
Taxonomy assignment approach determines the efficiency of identification of OTUs in marine nematodes
Autor/-innen des ZFMK: 
Publiziert in: 
Royal Society Open Science
Publikationstyp: 
Zeitschriftenaufsatz
DOI Name: 
10.1098/rsos.170315
Keywords: 
biodiversity; identification; barcode; nematodes; metabarcoding; meiobenthos
Bibliographische Angaben: 
Holovachov, O., Haenel, Q., Bourlat, S. J., Jondelius, U. (2017): Taxonomy assignment approach determines the efficiency of identification of OTUs in marine nematodes. Royal Society Open Science 4 (8): 170315. https://dx.doi.org/10.1098/rsos.170315
Abstract: 

Precision and reliability of barcode-based biodiversity assessment can be affected at several steps during acquisition and analysis of data. Identification of operational taxonomic units (OTUs) is one of the crucial steps in the process and can be accomplished using several different approaches, namely, alignment-based, probabilistic, tree-based and phylogenybased. The number of identified sequences in the reference databases affects the precision of identification. This paper compares the identification of marine nematode OTUs using alignment-based, tree-based and phylogeny-based approaches. Because the nematode reference dataset is limited in its taxonomic scope, OTUs can only be assigned to higher taxonomic categories, families. The phylogeny-based approach using the evolutionary placement algorithm provided the largest number of positively assigned OTUs and was least affected by erroneous sequences and limitations of reference data, compared to alignment-based and tree-based approaches.