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Preparation of amplicon libraries for metabarcoding of marine eukaryotes using Illumina MiSeq: the dual- PCR method

AutorInnen: 
Bourlat, S. J., Haenel, Q., Finnman, J., Leray, M.
Erscheinungsjahr: 
2016
Vollständiger Titel: 
Preparation of amplicon libraries for metabarcoding of marine eukaryotes using Illumina MiSeq: the dual- PCR method
Autor/-innen des ZFMK: 
Publiziert in: 
Methods in Molecular Biology
Publikationstyp: 
Beitrag zu Sammelwerk
DOI Name: 
10.1007/978-1-4939-3774-5_13
Keywords: 
Metabarcoding; Biodiversity; Microscopic eukaryotes; Illumina MiSeq
Bibliographische Angaben: 
Bourlat, S. J., Haenel, Q., Finnman, J., Leray, M. (2016): Preparation of amplicon libraries for metabarcoding of marine eukaryotes using Illumina MiSeq: the dual- PCR method. In: Bourlat, S. J. (ed.): Marine Genomics - Methods and protocols. - Springer (New York). pp. 197-208. https://doi.org/10.1007/978-1-4939-3774-5_13
Abstract: 

This protocol details the preparation of multiplexed amplicon libraries for metabarcoding (amplicon-based) studies of microscopic marine eukaryotes. Metabarcoding studies, based on the amplification of a taxonomically informative marker from a collection of organisms or an environmental sample, can be performed to analyze biodiversity patterns or predator–prey interactions. For Metazoa, we use the mitochondrial cytochrome oxidase 1 (CO1) or the small ribosomal subunit (SSU) markers. Here, we describe a strategy for the preparation of multiplexed Illumina MiSeq libraries using a dual-PCR approach for the addition of index and adaptor sequences.